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KMID : 1041219880300020096
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Journal of Sericultural and Entomological Science
1988 Volume.30 No. 2 p.96 ~ p.104
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Purification and Immunological Properties of Vitellin, and its Quantitative Changes during Embyrogenesis in the silkworm, Bombyx mori
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Abstract
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Vitellin, the major yolk protein of the silkworm, Bombyx mori was purified, and its immunological properties and the quantitative changes during embryogenesis were studied. The ovary transplantation into male hosts was also carried out to find out its effect on the yolk protein synth esis.
The pupal vitellogenin and the egg vitellin of Bombyx mori were purified by DEAE-cellulose column chromatography. These two female specific proteins showed the same mobility in the polyacrylamide gel electrophoresis and the same reaction in the double immunodiffusion test. The immunological identity was also observed between the vitellins of Bombyx mori and Bombyx mandarina.
The rudimentary ovaries transplanted into the male hosts of silkworms produced eggs without vitellin, indicating that the yolk precursors synthesized in other female organ beyond the ovary were necessary to produce vitellins. The major yolk protein, vitellin was disintegrated and utilized mostly during late stage of embryogenesis. It was different characteristics from the egg specific protein, which was utilized continuously from the early embryonic stage.
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